ID | JOS-0258851X-28-785 |
著者:名前 | Ohno, Hirokazu/ Miyoshi, Shozo/ Araho, Daisuke/ 金本, 大成/ 寺久保, 繁美/ 中島, 秀喜/ 津田, 整/ 須永, 克佳/ 天野, 滋/ Ohkoshi, Emika/ 坂上, 宏/ 佐藤, 和恵/ Yamamoto, Masaji |
著者:別形式 | Ohno, Hirokazu / Miyoshi, Shozo / Araho, Daisuke / Kanamoto, Taisei / Terakubo, shigemi / Nakashima, hideki / Tsuda, Tadashi / Sunaga, Katsuyoshi / Amano, Shigeru / Ohkoshi, Emika / Sakagami, Hiroshi / Satoh, Kazue / Yamamoto, Masaji |
著者:カナ | オオノ, ヒロカズ/ ミヨシ, ショウゾウ/ アラホ, ダイスケ/ カナモト, タイセイ/ テラクボ, シゲミ/ ナカシマ, ヒデキ/ ツダ, タダシ/ スナガ, カツヨシ/ アマノ, シゲル/ オオコシ, エミカ/ サカガミ, ヒロシ/ サトウ, カズエ/ ヤマモト, マサジ |
著者:所属 | 丸善製薬株式会社 / 丸善製薬株式会社 / 丸善製薬株式会社 / 聖マリアンナ医科大学 / 聖マリアンナ医科大学 / 聖マリアンナ医科大学/ 城西大学薬学部 / 城西大学薬学部 / 明海大学歯学部 / 明海大学歯学部 / 明海大学歯学部 / 明海大学歯学部 / 丸善製薬株式会社 |
著者:所属(別形式) | Maruzen Pharmaceuticals Co. Ltd. / Maruzen Pharmaceuticals Co. Ltd. / Maruzen Pharmaceuticals Co. Ltd. / St. Marianna University School of Medicine / St. Marianna University School of Medicine / St. Marianna University School of Medicine / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Meikai University School of Dentistry / Meikai University School of Dentistry / Meikai University School of Dentistry / Meikai University School of Dentistry / Maruzen Pharmaceuticals Co. Ltd. |
著者版フラグ | publisher |
出版地 | Athens |
出版者 | International Institute of Anticancer Research |
NCID | AA10714348 |
冊子ISSN | 0258851X |
掲載誌名 | |
巻 | 28 |
号 | 5 |
刊行年月 | 2014-09 |
開始ページ | 785 |
終了ページ | 794 |
コンテンツ作成日 | 2014-07-07 |
コンテンツ登録日 | 2014-09-08 |
抄録 | Compared to studies of water extracts of plants, those utilising alkaline extracts are limited. Both water and alkaline extracts from licorice root were compared regarding their biological activities. Licorice root was successively extracted first with water or alkaline solution (pH 9 or 12), and the alkaline (pH 12.0) extract was further separated into 50% ethanol-soluble and -insoluble fractions. Viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide method. Antibacterial activity against Porphyromonas gingivalis 381 was determined by turbidity assay. Cytochrome P-450 (CYP)3A4 activity was measured by β-hydroxylation of testosterone using human recombinant CYP3A4. Radical intensity of superoxide and hydroxyl radicals was determined by electron spin resonance spectroscopy. Alkaline extraction yielded slightly higher amounts of dried materials compared to water extraction. Alkaline extract showed higher anti-HIV and antibacterial activities, and similar magnitudes of CYP3A4 inhibitory and superoxide and hydroxyl radical-scavenging activities, compared to water extract. When alkaline extract was fractionated by 50% ethanol, anti-HIV activity was recovered from the insoluble fraction representing approximately 3% of the alkaline extract, whereas antibacterial activity was concentrated in the soluble fraction rich in glycyrrhizid acid, flavanones and chalcones. All extracts and sub-fractions led to bimodal hormetic dose-response (maximum hormetic response=238%) on the bacterial growth. The present study demonstrated the superiority of alkaline extraction over water extraction for preparing anti-HIV and antibacterial agents at higher yield from licorice root. |
キーワード | |
言語 | eng |
資源タイプ | text |
ジャンル | |
フォーマット | application/pdf |
権利 | Copyright c2014 International Institute of Anticancer Research |
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