| 著者：別形式 | Mitsumori, Akiho / Narumi, Sachie / Inoue, Yutaka / Murata, Isamu / Mitomo, Shun-ichi / Negishi, Yukiko / Kanamoto, Ikuo |
| 著者：所属(別形式） | Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Laboratory of Drug Safety Management / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Laboratory of Drug Safety Management / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Laboratory of Drug Safety Management / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Laboratory of Drug Safety Management / Kagawa Nutrition University, Institute of Nutrition Science / Kagawa Nutrition University, Institute of Nutrition Science / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Laboratory of Drug Safety Management |
| 抄録 | The use of an electrochemical detector (ECD) and core-shell columns as a new quantitative method for the analysis of α-, β-, and γ-cyclodextrin(αCD, βCD, and γCD, respectively) was investigated, and simple quantitative methods and appropriate measurement conditions were developed. The retention times of αCD, βCD, and γCD were determined to be 6.5, 12.9, and 10.4 min, respectively, when measured using an ECD, a 50°C column temperature, 0.5 mL/ min flow rate, 20 µL injection volume, 0.3 mol/L NaOH/0.3 mol/L CH3 COONa=1/1 mobile phase, and an S-40/60=St (styrene)/DVB (divinylbenzene)-5TMA (trimethylamine) core-shell column. The calibration curves of αCD, βCD, and γCD demonstrated good linearity (R2 >0.998). The repeatability, precision, and accuracy were acceptable (relative standard deviation <5% and Recovery=100 ± 5%). The limit of detection and quantification of αCD were determined to be 0.26 and 0.81 µg/mL, those of βCD were 7.65 and 23.19 µg/mL, and those of γCD were 3.87 and 11.74 µg/mL, respectively. Thus, analysis of the cyclodextrins αCD, βCD, and γCD could be carried out by a simple method using an ECD and a core-shell column. |
| 権利 | Copyright © 2018 Mitsumori A, et al. This is an open-access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
