ID JOS-analsci.28.621
著者:名前 著者:別形式 Yamazaki, Kenichi / Ikeguchi, Yoshihiko / Niwa, Takuya / Hayashi, Kaoru / Iwaki, Takahiro / Ishii, Ikumi / Niitsu, Masaru / Pegg, Anthony E. / Shirahata, Akira
著者:カナ 著者:所属 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / Pennsylvania State University College of Medicine / 城西大学薬学部
著者:所属(別形式) Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Pennsylvania State University College of Medicine / Josai University, Faculty of Pharmaceutical Sciences
著者版フラグ publisher
出版地 東京
出版者 日本分析化学会
出版者:カナ ニホンブンセキカガッカイ
出版者:別名 The Japan Society for Analytical Chemistry
NCID AA10500785
冊子ISSN 09106340
電子ISSN 13482246
掲載誌名 巻 28
号 6
刊行年月 2012-06
開始ページ 621
終了ページ 624
コンテンツ作成日 2012-04-10
コンテンツ登録日 2012-12-11
識別番号:DOI info:doi/10.2116/analsci.28.621
識別番号:DOI(リンク) 識別番号:その他 JOI:DN/JST.JSTAGE/analsci/28.621
抄録 Polyamines such as spermidine (Spd) and spermine (Spm), produced by aminopropyltransferase (Apt), play roles in cell growth and differentiation. A sensitive and simple fluorometric high-performance liquid chromatographic determination for Apt activity of spermidine synthase (Spdsyn) and spermine synthase (Spmsyn) was developed in order to examine cellular functions of polyamine synthesis. The derivatization procedure for methylthioadenosine (MTA) produced from decarboxylated S-adenosylmethionine by Apt was the reaction with 2-chloroacetaldehyde to give fluorescent 1, N6-etheno methylthioadenosine. The reaction conditions for derivatization were optimized. A calibration curve was established, ranging from 0.01 to 25 pmol. Quantification of derivatized MTA was confirmed to be identical to Spd or Spm production. The developed method determined Spdsyn and Spmsyn activities in HepG2 cells treated with oleic acid as a cellular lipid accumulation model.
キーワード 言語 eng
資源タイプ text
ジャンル フォーマット application/pdf
このアイテムを表示する:本文(pdf) ( 575.5KB) ダウンロード回数:
回
このアイテムを表示する:URI