ID | JOS-bpb.28.2271 |
著者:名前 | |
著者:別形式 | Kawata, Takeshi / Homma, Masato / Kakiuchi, Yoshihiro / Inomata, Shinichi / Miyabe, Masayuki / Kobayashi, Daisuke / Morimoto, Yasunori / Kohda, Yukinao |
著者:カナ | |
著者:所属 | 筑波大学 / 筑波大学 / 筑波大学附属病院 / 筑波大学 / 筑波大学 / 城西大学薬学部 / 城西大学薬学部 / 筑波大学 |
著者:所属(別形式) | University of Tsukuba, Graduate School of Comprehensive Human Sciences, Department of Pharmaceutical Sciences / University of Tsukuba, Graduate School of Comprehensive Human Sciences, Department of Pharmaceutical Sciences / Tsukuba University Hospital, Department of Pharmacy / University of Tsukuba, Graduate School of Comprehensive Human Sciences, Department of Pharmaceutical Sciences / University of Tsukuba, Graduate School of Comprehensive Human Sciences, Department of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / University of Tsukuba, Graduate School of Comprehensive Human Sciences, Department of Pharmaceutical Sciences |
著者版フラグ | publisher |
出版地 | 東京 |
出版者 | 日本薬学会 |
出版者:カナ | ニホンヤクガクカイ |
出版者:別名 | The Pharmaceutical Society of Japan |
NCID | AA10885497 |
冊子ISSN | 0918-6158 |
電子ISSN | 1347-5215 |
掲載誌名 | |
巻 | 28 |
号 | 12 |
刊行年月 | 2005-12 |
開始ページ | 2271 |
終了ページ | 2273 |
コンテンツ作成日 | 2005-07-15 |
コンテンツ修正日 | 2005-10-04 |
コンテンツ登録日 | 2018-09-27 |
識別番号:DOI | info:doi/10.1248/bpb.28.2271 |
識別番号:DOI(リンク) | |
NII論文ID | 110004049130 |
医中誌ID | 2006248211 |
抄録 | We developed assay method for determination of plasma ropivacaine by using reversed-phase high performance liquid chromatography (HPLC) equipped with ordinary octadecylsilyl silica-gel (ODS) column. Plasma samples spiked with internal standard (bupivacaine) were treated by ethylacetate to extract ropivacaine and internal standard. The ropivacaine and internal standard separated on ODS column were detected by an ultra violet (UV) detector set at 215 nm. The mobile phase solvent consisted of acetonitrile, methanol and 0.05 M phosphate buffer adjusted to pH 4.0 (10 : 30 : 60, v/v) was pumped at a flow rate of 0.8 ml/min. The calibration curve of ropivacaine was linear at the concentration of 25—1000 ng/ml (r=0.9998). The recoveries of ropivacaine from plasma were greater than 87.9% with the coefficient of variations (CVs) less than 6.1%. The CVs for intra- and inter-day assay of ropivacaine were 2.0—12.0% and 1.7—14.8%, respectively. This HPLC method was applied to determining plasma ropivacaine in two healthy subjects after receiving 0.5% ropivacaine viscous preparation, which was prepared in our hospital. Our preliminary pharmacokinetic data showed that ropivacaine viscous could be used safely based on the plasma ropivacaine concentrations (Cmax: 89—125 ng/ml) for pain relief in oral mucosa. |
キーワード | |
言語 | eng |
資源タイプ | text |
ジャンル | |
フォーマット | application/pdf |
権利 | Copyright © 2005 Pharmaceutical Society of Japan |
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