城西大学機関リポジトリJURASugar-Binding Properties of the Two Lectin Domains of LEC-1 with Respect to the Galβ1-4Fuc Disaccharide Unit Present in Protostomia Glycoconjugates

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Sugar-Binding Properties of the Two Lectin Domains of LEC-1 with Respect to the Galβ1-4Fuc Disaccharide Unit Present in Protostomia Glycoconjugates

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ID	JOS-bpb.34.1134
著者：名前	武内, 智春/Sugiura, Ken-ichi/西山, 和沙/高橋, 秀依/夏苅, 英昭/荒田, 洋一郎/Kasai, Ken-ichi
著者：別形式	Takeuchi, Tomoharu / Sugiura, Ken-ichi / Nishiyama, Kazusa / Takahashi, Hideyo / Natsugari, Hideaki / Arata, Yoichiro / Kasai, Ken-ichi
著者：カナ	タケウチ, トモハル/スギウラ, ケンイチ/ニシヤマ, カズサ/タカハシ, ヒデヨ/ナツガリ, ヒデアキ/アラタ, ヨウイチロウ/カサイ, ケンイチ
著者：所属	城西大学薬学部 / 帝京大学薬学部 / 帝京大学薬学部 / 帝京大学薬学部 / 帝京大学薬学部 / 城西大学薬学部 / 帝京大学薬学部
著者：所属(別形式）	Josai University, Faculty of Pharmaceutical Science, Laboratory of Molecular Immunology / Teikyo University, School of Pharmaceutical Sciences, Department of Biological Chemistry / Teikyo University, School of Pharmaceutical Sciences, Laboratory of Synthetic Organic and Medicinal Chemistry / Teikyo University, School of Pharmaceutical Sciences, Laboratory of Synthetic Organic and Medicinal Chemistry / Teikyo University, School of Pharmaceutical Sciences, Laboratory of Synthetic Organic and Medicinal Chemistry / Josai University, Faculty of Pharmaceutical Science, Laboratory of Molecular Immunology / Teikyo University, School of Pharmaceutical Sciences, Department of Biological Chemistry
著者版フラグ	publisher
出版地	東京
出版者	日本薬学会
出版者：カナ	ニホンヤクガクカイ
出版者：別名	The Pharmaceutical Society of Japan
NCID	AA10885497
冊子ISSN	09186158
電子ISSN	13475215
掲載誌名	Biological and Pharmaceutical Bulletin
巻	34
号	7
刊行年月	2011-07
開始ページ	1134
終了ページ	1138
コンテンツ作成日	2011-04-14
コンテンツ登録日	2013-07-16
識別番号：DOI	info:doi/10.1248/bpb.34.1134
識別番号：DOI（リンク）	http://dx.doi.org/10.1248/bpb.34.1134
識別番号：その他	JOI:JST.JSTAGE/bpb/34.1134
抄録	Galβ1-4Fuc disaccharide unit was recently reported to be the endogenous structure recognized by the galectin LEC-6 isolated from the nematode Caenorhabditis elegans. LEC-1, which is another major galectin from this organism, is a tandem repeat-type galectin that contains two carbohydrate recognition domains, the N-terminal lectin domain (LEC-1Nh) and the C-terminal lectin domain (LEC-1Ch), and was also found to have an affinity for the Galβ1-4Fuc disaccharide unit. In the present study, we compared the binding strengths of LEC-1, LEC-1Nh, and LEC-1Ch to Galβ1-4Fuc, Galβ1-3Fuc, and Galβ1-4GlcNAc units as well as to LEC-6-ligand N-glycans by using frontal affinity chromatography (FAC) analysis. The two lectin domains of LEC-1 exhibited the highest affinity for Galβ1-4Fuc, though sugar-binding properties differed somewhat between LEC-1Nh and LEC-1Ch. Furthermore, these two domains had significantly lower affinities for the LEC-6-binding glycans. These results suggest that the endogenous recognition unit of LEC-1 is likely to be Galβ1-4Fuc, and that the endogenous ligands for LEC-1 are different from those for LEC-6.
キーワード	Caenorhabditis elegans/galectin/frontal affinity chromatography/Galβ1-4Fuc/Galβ1-4GlcNAc/LEC-1
言語	eng
資源タイプ	text
ジャンル	01学術雑誌論文 / Journal Article
フォーマット	application/pdf
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このアイテムを表示する：URI	http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-bpb.34.1134

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