城西大学機関リポジトリJURASerotonin Suppresses β-Casein Expression via Inhibition of the Signal Transducer and Activator of Transcription 5 (STAT5) Protein Phosphorylation in Human Mammary Epithelial Cells MCF-12A

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Serotonin Suppresses β-Casein Expression via Inhibition of the Signal Transducer and Activator of Transcription 5 (STAT5) Protein Phosphorylation in Human Mammary Epithelial Cells MCF-12A

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ID	JOS-bpb.37.1336
著者：名前	千葉, 健史/木村, 聡一郎/高橋, 勝雄/森本, 雍憲/三部, 篤/上田, 秀雄/工藤, 賢三
著者：別形式	Chiba, Takeshi / Kimura, Soichiro / Takahashi, Katsuo / Morimoto, Yasunori / Sanbe, Atsushi / Ueda, Hideo / Kudo, Kenzo
著者：カナ	チバ, タケシ/キムラ, ソウイチロウ/タカハシ, カツオ/モリモト, ヤスノリ/サンベ, アツシ/ウエダ, ヒデオ/クドウ, ケンゾウ
著者：所属	岩手医科大学 / 城西大学薬学部 / 岩手医科大学 / 城西大学薬学部 / 岩手医科大学 / 城西大学薬学部 / 岩手医科大学
著者：所属(別形式）	Iwate Medical University / Josai University, Faculty of Pharmaceutical Sciences / Iwate Medical University / Josai University, Faculty of Pharmaceutical Sciences / Iwate Medical University / Josai University, Faculty of Pharmaceutical Sciences / Iwate Medical University
著者版フラグ	publisher
出版地	東京
出版者	日本薬学会
出版者：カナ	ニホンヤクガクカイ
出版者：別名	The Pharmaceutical Society of Japan
NCID	AA10885497
冊子ISSN	0918-6158
電子ISSN	1347-5215
掲載誌名	Biological and Pharmaceutical Bulletin
巻	37
号	8
刊行年月	2014-08
開始ページ	1336
終了ページ	1340
コンテンツ作成日	2014-03-31
コンテンツ修正日	2014-05-16
コンテンツ登録日	2018-09-27
識別番号：DOI	info:doi/10.1248/bpb.b14-00273
識別番号：DOI（リンク）	http://doi.org/10.1248/bpb.b14-00273
NII論文ID	130004677543
医中誌ID	2015097889
抄録	Serotonin (5-hydroxytryptamine; 5-HT) has an important physiological role in controlling lactation, namely, milk volume homeostasis, within mammary glands. The objectives of this study were to evaluate whether exogenous 5-HT can suppress β-casein expression, a differentiation marker, produced in human mammary epithelial cells, and to determine whether 5-HT can attenuate β-casein signaling via the prolactin (PRL) receptor (PRLr) and Janus kinase 2/signal transducer and activator of transcription 5 (STAT5) pathway. PRL treatment increased the mRNA level of β-casein in the MCF-12A human mammary epithelial cell line, and the highest level occurred at days 7 and 14 of culture. In contrast, PRLr expression was not affected significantly by PRL treatment. PRL treatment in MCF-12A cells increased levels of β-casein and phosphorylated STAT5 (pSTAT5) proteins in a concentration-dependent manner, with a slight increase of STAT5 protein. β-Casein expression was inhibited by 0.1 mM 5-HT in a time-dependent manner. Additionally, treatment with 0.1 mM 5-HT for 72 h decreased protein levels of β-casein and pSTAT5, with a slight decrease in STAT5 levels. These results suggest that exogenous 5-HT can inhibit STAT5 phosphorylation, resulting in a decrease in β-Casein expression. In conclusion, we showed that exogenous 5-HT decreased β-casein expression in MCF-12A human mammary epithelial cells, and that 5-HT was responsible for inhibiting phosphorylation of STAT5, resulting in a decline in lactational function.
キーワード	serotonin/β-casein/human mammary gland/MCF-12A/milk protein
言語	eng
資源タイプ	text
ジャンル	01学術雑誌論文 / Journal Article
フォーマット	application/pdf
権利	Copyright © 2014 The Pharmaceutical Society of Japan
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このアイテムを表示する：URI	http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-bpb.37.1336

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