| ID | JOS-bpb.b12-00818 |
| 著者：名前 | |
| 著者：別形式 | Takao, Koichi / Shirahata, Akira / Samejima, Keijiro / Casero, Robert Anthony Jr. / Igarashi, Kazuei / Sugita, Yoshiaki |
| 著者：カナ | |
| 著者：所属 | 城西大学薬学部 / 城西大学薬学部 / 東京都臨床医学総合研究所 / Johns Hopkins University, Department of Oncology, School of Medicine / 千葉大学 / 城西大学薬学部 |
| 著者：所属(別形式） | Josai University, Department of Pharmaceutical Technochemistry, Laboratory of Bioorganic Chemistry / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Tokyo Metropolitan Institute of Medical Science, Department of Molecular Medicine / Johns Hopkins University, Department of Oncology, School of Medicine / Chiba University, Graduate School of Pharmaceutical Sciences / Josai University, Department of Pharmaceutical Technochemistry, Laboratory of Bioorganic Chemistry |
| 著者版フラグ | publisher |
| 出版地 | 東京 |
| 出版者 | 日本薬学会 |
| 出版者：カナ | ニホンヤクガクカイ |
| 出版者：別名 | The Pharmaceutical Society of Japan |
| NCID | AA10885497 |
| 冊子ISSN | 09186158 |
| 電子ISSN | 13475215 |
| 掲載誌名 | |
| 巻 | 36 |
| 号 | 3 |
| 刊行年月 | 2013-03 |
| 開始ページ | 407 |
| 終了ページ | 411 |
| コンテンツ作成日 | 2012-12-02 |
| コンテンツ登録日 | 2013-07-18 |
| 識別番号：DOI | info:doi/10.1248/bpb.b12-00818 |
| 識別番号：DOI（リンク） | |
| 識別番号：その他 | JOI:DN/JST.JSTAGE/bpb/b12-00818 |
| 抄録 | Substrate activities of various linear polyamines to human spermine oxidase (hSMO) were investigated. The activities were evaluated by monitoring the amount of H2O2 released from sample polyamines by hSMO. H2O2 was measured by a HPLC method that analyzed fluorescent dimers derived from the oxidation of homovanillic acid in the presence of horseradish peroxidase. Six triamines were tested and were found not to be hSMO substrates. Of sixteen tetramines tested, spermine (Spm) was the most active substrate, followed by homospermine and N-butylated Spm. Pentamines showed a characteristic pattern of substrate activity. Of thirteen pentamines tested, 3343 showed higher substrate activity than Spm, and 4343 showed similar activity to Spm. The activities of the other pentamines were as follows: 3443, 4443, 4344, 3344, 4334, 4444, and 3334 (in decreasing order). Product amines released from these pentamines by hSMO were then analyzed by HPLC. Triamine was the only observed product, and the amount of triamine was nearly equivalent to that of released H2O2. A marked difference in the pH dependency curves between tetramines and pentamines suggested that hSMO favored reactions with a non-protonated secondary nitrogen at the cleavage site. The Km and Vmax values for Spm and 3343 at pH 7.0 and 9.0 were consistent with the higher substrate activity of 3343 compared to Spm, as well as with the concept of a non-protonated secondary nitrogen at the cleavage site being preferred, and 3343 was well degraded at a physiological pH by hSMO. |
| キーワード | |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| このアイテムを表示する：本文(pdf) |  ( 516.5KB) ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
