城西大学機関リポジトリJURAPoly-L-arginine-Induced Internalization of Tight Junction Proteins Increases the Paracellular Permeability of the Caco-2 Cell Monolayer to Hydrophilic Macromolecules

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Poly-L-arginine-Induced Internalization of Tight Junction Proteins Increases the Paracellular Permeability of the Caco-2 Cell Monolayer to Hydrophilic Macromolecules

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ID	JOS-bpb.b12-00878
著者：名前	八巻, 努/大竹, 一男/市川, 景子/内田, 昌希/内田, 博之/大島, 新司/從二, 和彦/小林, 順/森本, 雍憲/夏目, 秀視
著者：別形式	Yamaki, Tsutomu / Ohtake, Kazuo / Ichikawa, Keiko / Uchida, Masaki / Uchida, Hiroyuki / Oshima, Shinji / juni, Kazuhiko / Kobayashi, Jun / Morimoto, Yasunori / Natsume, Hideshi
著者：カナ	ヤマキ, ツトム/オオタケ, カズオ/イチカワ, ケイコ/ウチダ, マサキ/ウチダ, ヒロユキ/オオシマ, シンジ/ジュウニ, カズヒコ/コバヤシ, ジュン/モリモト, ヤスノリ/ナツメ, ヒデシ
著者：所属	城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部
著者：所属(別形式）	Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences
著者版フラグ	publisher
出版地	東京
出版者	日本薬学会
出版者：カナ	ニホンヤクガクカイ
出版者：別名	The Pharmaceutical Society of Japan
NCID	AA10885497
冊子ISSN	09186158
電子ISSN	13475215
掲載誌名	Biological and Pharmaceutical Bulletin
巻	36
号	3
刊行年月	2013-03
開始ページ	432
終了ページ	441
コンテンツ作成日	2012-12-15
コンテンツ修正日	2014-01-31
コンテンツ登録日	2013-07-18
識別番号：DOI	info:doi/10.1248/bpb.b12-00878
識別番号：DOI（リンク）	http://dx.doi.org/10.1248/bpb.b12-00878
識別番号：その他	JOI:DN/JST.JSTAGE/bpb/b12-00878
抄録	We investigated whether poly-L-arginine (PLA) enhances the paracellular permeability of the Caco-2 monolayer to hydrophilic macromolecules and clarified the disposition of tight junction (TJ) proteins. The transepithelial electrical resistance (TEER) and fluorescein isothiocyanate (FITC)-dextran (FD-4) permeation were determined after treatment with PLA. TJ proteins were visualized using immunofluorescence microscopy after PLA exposure and depletion, and their expression levels were determined. The barrier function of TJs was also evaluated by measuring the alterations in the TEER and in the localization of TJ proteins. PLA induced an increase in hydrophilic macromolecule, FD-4, permeation through Caco-2 cell monolayers and a decrease in the TEER in a concentration-dependent manner, without any significant impact on the cell viability. This increased paracellular permeability induced by PLA was found to be internalized of claudin-4, ZO-1, tricellulin and mainly occludin from cell?cell junction to the subcellular space. ZO-1 appeared to play an important role in the reconstitution of TJ strand structures following PLA depletion. These results indicate that the PLA led to the internalization of TJ proteins to the subcellular space, subsequently increasing the permeability of the Caco-2 cell monolayer to FD-4 via a paracellular route.
キーワード	poly-L-arginine/absorption enhancer/drug delivery/hydrophilic-macromolecule/tight junction
注記	Errata: Biological and Pharmaceutical Bulletin Vol. 37 (2014) No. 1 p. 184. Released on J-STAGE 20140101. Author name : Shinji Ohshima → Shinji Oshima. / http://dx.doi.org/10.1248/bpb.Errata
言語	eng
資源タイプ	text
ジャンル	01学術雑誌論文 / Journal Article
フォーマット	application/pdf
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このアイテムを表示する：URI	http://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-bpb.b12-00878

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