| ID | JOS-bpb.b13-00631 |
| 著者：名前 | |
| 著者：別形式 | Karahashi, Minako / Fukuhara, Hiroto / Hoshina, Miki / Sakamoto, Takeshi / Yamazaki, Tohru / Mitsumoto, Atsushi / Kawashima, Yoichi / Kudo, Naomi |
| 著者：カナ | |
| 著者：所属 | 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西国際大学薬学部 / 城西大学薬学部 / 城西大学薬学部 |
| 著者：所属(別形式） | Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai International University, Faculty of Pharmaceutical Sciences/ Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences |
| 著者版フラグ | publisher |
| 出版地 | 東京 |
| 出版者 | 日本薬学会 |
| 出版者：カナ | ニホンヤクガクカイ |
| 出版者：別名 | The Pharmaceutical Society of Japan |
| NCID | AA10885497 |
| 冊子ISSN | 09186158 |
| 電子ISSN | 13475215 |
| 掲載誌名 | |
| 巻 | 37 |
| 号 | 1 |
| 刊行年月 | 2014-01 |
| 開始ページ | 105 |
| 終了ページ | 112 |
| コンテンツ作成日 | 2013-10-18 |
| コンテンツ登録日 | 2014-03-11 |
| 識別番号：DOI | info:doi/10.1248/bpb.b13-00631 |
| 識別番号：DOI（リンク） | |
| 識別番号：その他 | JOI:DN/JST.JSTAGE/bpb/b13-00631 |
| 抄録 | Fibrates are used in biochemical and pharmacological studies as bioactive tools. Nevertheless, most studies have lacked information concerning the concentrations of fibric acids working inside tissues because a simple and sensitive method is not available for their quantitation. This study aimed to develop a simple and sensitive bioanalytical method for the quantitation of clofibric, bezafibric and fenofibric acids in samples of very small portions of tissues. Fibric acids were extracted into n-hexane?ethyl acetate from tissue homogenates (10?mg of liver, kidney or muscle) or serum (100??L) and were derivatized with 4-bromomethyl-6,7-dimethoxycoumarin, followed by HPLC with fluorescence detection. These compounds were separated isocratically on a reversed phase with acetonitrile?water. Standard analytical curves were linear over the concentration range of 0.2?20?nmol/10?mg of liver. Precision and accuracy were within acceptable limits. Recovery from liver homogenates ranged from 93.03 to 112.29%. This method enabled the quantitation of fibric acids in 10?mg of liver from rats treated with clofibric acid, bezafibric acid or fenofibrate. From these analytical data, it became clear that there was no large difference in ratio of acyl-CoA oxidase 1 (Acox1) mRNA level to fibric acid content in the liver among the three fibric acids, suggesting that these three fibric acids have similar potency to increase expression of the Acox1 gene, which is a target of peroxisome proliferator-activated receptor α. Thus, the proposed method is a simple, sensitive and reliable tool for the quantitation of fibric acids working in vivo inside livers. |
| キーワード | |
| 注記 | Regular Articles |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | Copyright c 2014 by the PHARMACEUTICAL SOCIETY OF JAPAN |
| このアイテムを表示する：本文(pdf) |  ( 730.2KB) ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
