| ID | JOS-bpb.b15-00735 |
| 著者：名前 | |
| 著者：別形式 | Naito, Kota / Tanaka, Chizuru / Mitsuhashi, Manami / Moteki, Hajime / Kimura, Mitsutoshi / Natsume, Hideshi / Ogihara, Masahiko |
| 著者：カナ | |
| 著者：所属 | 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 |
| 著者：所属(別形式） | Josai University, Faculty of Pharmaceutical Science, Department of Clinical Pharmacology / Josai University, Faculty of Pharmaceutical Science, Department of Clinical Pharmacology / Josai University, Faculty of Pharmaceutical Science, Department of Pharmaceutics / Josai University, Faculty of Pharmaceutical Science, Department of Pharmaceutics / Josai University, Faculty of Pharmaceutical Science, Department of Clinical Pharmacology / Josai University, Faculty of Pharmaceutical Science, Department of Pharmaceutics / Josai University, Faculty of Pharmaceutical Science, Department of Clinical Pharmacology |
| 著者版フラグ | publisher |
| 出版地 | 東京 |
| 出版者 | 日本薬学会 |
| 出版者：カナ | ニホンヤクガクカイ |
| 出版者：別名 | The Pharmaceutical Society of Japan |
| NCID | AA10885497 |
| 冊子ISSN | 09186158 |
| 電子ISSN | 13475215 |
| 掲載誌名 | |
| 巻 | 39 |
| 号 | 1 |
| 刊行年月 | 2016-01 |
| 開始ページ | 121 |
| 終了ページ | 126 |
| コンテンツ作成日 | 2015-09-24 |
| コンテンツ修正日 | 2015-11-06 |
| コンテンツ登録日 | 2016-02-15 |
| 識別番号：DOI | info:doi/10.1248/bpb.b15-00735 |
| 識別番号：DOI（リンク） | |
| 抄録 | The involvement of serotonin (5-hydroxytryptamine; 5-HT) and the 5-HT2 receptor subtypes in the
induction of DNA synthesis and proliferation was investigated in primary cultures of adult rat hepatocytes
to elucidate the intracellular signal transduction mechanisms. Hepatocyte parenchymal cells maintained
in a serum-free, defined medium, synthesized DNA and proliferated in the presence of 5-HT or a selective
5-HT2B receptor agonist, BW723C86, but not in the presence of 5-HT2A, or 5-HT2C receptor agonists (TCB-2
and CP809101, respectively), in a time- and dose-dependent manner. A selective 5-HT2B receptor antagonist,
LY272015 (10 7 M), and a specific phospholipase C (PLC) inhibitor, U-73122 (10 6 M), as well as specific inhibitors
of growth-related signal transducers?including AG1478, LY294002, PD98059, and rapamycin?completely
inhibited 5-HT (10 6 M)- or BW723C86 (10 6 M)-induced hepatocyte DNA synthesis and proliferation.
Both 5-HT and BW723C86 were shown to significantly stimulate the phosphorylation of epidermal growth
factor (EGF)/transforming growth factor (TGF)-α receptor tyrosine kinase (p175 kDa) and extracellular
signal-regulated kinase (ERK) 2 on Western blot analysis. These results suggest that the proliferative mechanism
of activating 5-HT is mediated mainly through 5-HT2B receptor-stimulated Gq/PLC and EGF/TGF-
α-receptor/phosphatidylinositol 3-kinase (PI3K)/ERK2/mammalian target of rapamycin (mTOR) signaling
pathways in primary cultured hepatocytes. |
| キーワード | |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | Copyright c 2016 The Pharmaceutical Society of Japan |
| このアイテムを表示する：本文(pdf) |  ( 2.2MB) ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
