| ID | JOS-bpb.b18-00272 |
| 著者：名前 | |
| 著者：別形式 | Kitamura, Masashi / Aragane, Masako / Nakamura, Kou / Adachi, Tatsushi / Watanabe, Kazuhito / Sasaki, Yohei |
| 著者：カナ | |
| 著者：所属 | 金沢大学大学院医薬保健学総合研究科薬学専攻分子生薬学研究室 / 東京都健康安全研究センター薬用植物園 / 東京都健康安全研究センター薬用植物園 / 株式会社カネカ / 第一薬科大学 / 金沢大学大学院医薬保健学総合研究科薬学専攻分子生薬学研究室 |
| 著者：所属(別形式） | Kanazawa University, Graduate School of Medical Sciences, Division of Pharmaceutical Sciences, Laboratory of Molecular Pharmacognosy / Medicinal Plant Garden, Tokyo Metropolitan Institute of Public Health / Medicinal Plant Garden, Tokyo Metropolitan Institute of Public Health / Kaneka Corporation / Daiichi University of Pharmacy / Kanazawa University, Graduate School of Medical Sciences, Division of Pharmaceutical Sciences, Laboratory of Molecular Pharmacognosy |
| 著者版フラグ | publisher |
| 出版地 | 東京 |
| 出版者 | 日本薬学会 |
| 出版者：カナ | ニホンヤクガクカイ |
| 出版者：別名 | The Pharmaceutical Society of Japan |
| NCID | AA10885497 |
| 冊子ISSN | 09186158 |
| 電子ISSN | 13475215 |
| 掲載誌名 | |
| 巻 | 41 |
| 号 | 8 |
| 刊行年月 | 2018-8 |
| 開始ページ | 1303 |
| 終了ページ | 1306 |
| コンテンツ作成日 | 2018-04-09 |
| コンテンツ修正日 | 2018-05-12 |
| コンテンツ登録日 | 2019-02-15 |
| 識別番号：DOI | info:doi/10.1248/bpb.b18-00272 |
| 識別番号：DOI（リンク） | |
| PubMed番号 | 30068883 |
| 抄録 | Cannabis sativa L. is cultivated worldwide for a variety of purposes, but its cultivation and possession are regulated by law in many countries, necessitating accurate detection methods. We previously reported a DNA-based C. sativa identification method using the loop-mediated isothermal amplification (LAMP) assay. Although the LAMP technique can be used for on-site detection, our previous protocol took about 90 min from sampling to detection. In this study, we report an on-site protocol that can be completed in 30 min for C. sativa identification based on a modified LAMP system. Under optimal conditions, the LAMP reaction started at approximately 10 min and was completed within 20 min at 63°C. It had high sensitivity (10 pg of purified DNA). Its specificity for C. sativa was confirmed by examining 20 strains of C. sativa and 50 other species samples. With a simple DNA extraction method, the entire procedure from DNA extraction to detection required only 30 min. Using the protocol, we were able to identify C. sativa from various plant parts, such as the leaf, stem, root, seed, and resin derived from C. sativa extracts. As the entire procedure was completed using a single portable device and the results could be evaluated by visual detection, the protocol could be used for on-site detection and is expected to contribute to the regulation of C. sativa. |
| キーワード | |
| 注記 | Present address: Department of Pharmaceutical Sciences, Faculty of Pharmacy and Pharmaceutical Sciences, Josai University; 1–1 Keyakidai, Sakado, Saitama 350–0295, Japan. To whom correspondence should be addressed. e-mail: kitamura@josai.ac.jp |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | Copyright © 2018 Pharmaceutical Society of Japan |
| このアイテムを表示する：本文(pdf) |  ( 1.7MB) ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
