ID JOS-glycobcwp125
著者:名前 著者:別形式 Takeuchi, Tomoharu / Nishiyama, Kazusa / Sugiura, Ken-ichi / Takahashi, Miki / Yamada, Atsushi / Kobayashi, Susumu / Takahashi, Hideyo / Natsugari, Hideaki / Kasai, Ken-ichi
著者:カナ 著者:所属 帝京大学薬学部 / 帝京大学薬学部 / 帝京大学薬学部 / 帝京大学薬学部 / 帝京大学薬学部 / 東京理科大学薬学部 / 帝京大学薬学部 / 帝京大学薬学部 / 帝京大学薬学部
著者:所属(別形式) Teikyo University, School of Pharmaceutical Sciences / Teikyo University, School of Pharmaceutical Sciences / Teikyo University, School of pharmaceutical Sciences / Teikyo University, School of Pharmaceutical Sciences / Teikyo University, School of Pharmaceutical Sciences / TokyoUniversity of Science, Faculty of Pharmaceutical Sciences / Teikyo University, School of Pharmaceutical Sciences / Teikyo University, School of Pharmaceutical Sciences / Teikyo University, School of Pharmaceutical Sciences
著者版フラグ author
出版者 Oxford Journals
NCID AA10826501
冊子ISSN 09596658
電子ISSN 14602423
掲載誌名 巻 19
号 12
刊行年月 2009-08
開始ページ 1503
終了ページ 1510
コンテンツ作成日 2009-08-18
コンテンツ登録日 2011-02-08
識別番号:URI 識別番号:DOI info:doi/10.1093/glycob/cwp125
識別番号:DOI(リンク) PubMed番号 info:pmid/19690090
抄録 Galβ1-4GlcNAc is thought to be a common disaccharide unit preferentially recognized by vertebrate galectins. Eight amino acid residues conserved in proteins belonging to the galectin family have been suggested to be responsible for recognition. Meanwhile, we isolated and analyzed endogenous N-glycans of Caenorhabditis elegans that were captured by a C. elegans galectin LEC-6, and demonstrated that the unit of recognition for LEC-6 is a Gal-Fuc disaccharide, though the linkage between these residues was not confirmed. In the present study, we chemically synthesized Galβ1-4Fuc and Galβ1-3Fuc labeled with 2-aminopyridine (PA), and demonstrated that LEC-6 interacts with PA-Galβ1-4Fuc more strongly than PA-Galβ1-3Fuc by frontal affinity chromatography (FAC). Galβ1-4Fuc also inhibited hemagglutination caused by LEC-6 more strongly than Galβ1-3Fuc. FAC analysis using LEC-6 point mutants revealed that some of the conserved amino acid residues which have proved to be important for recognition of Galβ1-4GlcNAc are not necessary for the binding to Galβ1-4Fuc. Another major C. elegans galectin, LEC-1, also showed preferential binding toGalβ1-4Fuc. These results suggest that Galβ1-4Fuc is the endogenous unit structure recognized by C. elegans galectins, which implies that C. elegans glycans and galectins may have co-evolved through an alteration in the structures of C. elegans glycans and a subsequent conversion in the sugar-binding mechanism of galectins. Furthermore, since glycans containing the Galβ1-4Fuc disaccharide unit have been found in organisms belonging to 4 Protostomia, this unit might be a common glyco-epitope recognized by galectins in these organisms.
キーワード 注記 DOI: 10.1093/glycob/cwp125 竹内智春(Takeuchi, Tomoharu): 2010.4~ 城西大学(Josai University)薬学部薬学科分子免疫学講座
言語 eng
資源タイプ text
ジャンル フォーマット application/pdf
このアイテムを表示する:本文(pdf) ( 544.0KB) ダウンロード回数:
回
このアイテムを表示する:URI