| ID | JOS-j.bonr.2016.02.001 |
| 著者：名前 | |
| 著者：別形式 | Takeuchi, Tomoharu / Nagasaka, Moyuko / Shimizu, Miyuki / Tamura, Mayumi / Arata, Yoichiro |
| 著者：カナ | |
| 著者：所属 | 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 |
| 著者：所属(別形式） | Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / |
| 著者版フラグ | publisher |
| 出版者 | Elsevier |
| 電子ISSN | 23521872 |
| 掲載誌名 | |
| 巻 | 5 |
| 刊行年月 | 2016 |
| 開始ページ | 15 |
| 終了ページ | 21 |
| コンテンツ作成日 | 2016-01-07 |
| コンテンツ修正日 | 2016-02-01 |
| コンテンツ登録日 | 2016-03-04 |
| 識別番号：DOI | info:doi/10.1016/j.bonr.2016.02.001 |
| 識別番号：DOI（リンク） | |
| 抄録 | Osteoclasts are the only cells in an organism capable of resorbing bone. These cells differentiate from monocyte/macrophage lineage cells upon stimulation by receptor activator of NF-κB ligand (RANKL). On the other hand, osteoclastogenesis is reportedly suppressed by glucose via the downregulation of NF-κB activity through suppression of reactive oxygen species generation. To examine whether other sugars might also affect osteoclast development, we compared the effects of monomeric sugars (glucose, galactose, N-acetylglucosamine (GlcNAc), and N-acetylgalactosamine (GalNAc)) on the osteoclastogenesis of murine RAW264 cells. Our results demonstrated that, in addition to glucose, both GlcNAc and GalNAc, which each have little effect on the generation of reactive oxygen species, suppress osteoclastogenesis. We hypothesized that GlcNAc might affect osteoclastogenesis through the upregulation of O-GlcNAcylation and showed that GlcNAc increases global O-GlcNAcylation, thereby suppressing the RANKL-dependent phosphorylation of NF-κB p65. Furthermore, an inhibitor of N-acetyl-β-D-glucosaminidase, O-(2-acetamido-2-deoxy-D-glucopyranosylidene) amino N-phenylcarbamate (PUGNAc), which also increases O-GlcNAcylation, suppressed the osteoclastogenesis of RAW264 cells and that of human peripheral blood mononuclear cells. Together, these data suggest that GlcNAc suppresses osteoclast differentiation in part through the promotion of O-GlcNAcylation. |
| キーワード | |
| 注記 | A new online only, open access, peer reviewed journal. |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| 権利 | Copyright c2016 The Authors. Published by Elsevier Inc. |
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| このアイテムを表示する：URI | |
