ID JOS-j.ejphar.2012.12.010
著者：名前 著者：別形式 Moteki, Hajime / Kimura, Mitsutoshi / Sunaga, Katsuyoshi / Tsuda, Tadashi / Ogihara, Masahiko
著者：カナ 著者：所属 城西大学薬学部薬学科 / 城西大学薬学部薬学科 / 城西大学薬学部医療栄養学科薬物療法学 / 城西大学薬学部医療栄養学科薬物療法学 / 城西大学薬学部薬学科
著者：所属(別形式） Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Pharmacology /Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Pharmacology / Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Dietetics and Human Nutrition, Laboratory of Pharmacotherapy / Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Dietetics and Human Nutrition, Laboratory of Pharmacotherapy / Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Pharmacology
著者版フラグ author
出版者 Elsevier
NCID AA00639687
冊子ISSN 00142999
電子ISSN 00142999
掲載誌名 巻 700
号 1-3
刊行年月 2013-01
開始ページ 2
終了ページ 12
コンテンツ作成日 2012-12-07
コンテンツ登録日 2013-10-23
識別番号：DOI info:doi/10.1016/j.ejphar.2012.12.010
識別番号：DOI（リンク） 抄録 We investigated the effects of α- and β-adrenoceptor agonists on l-ascorbic acid-induced hepatocyte DNA synthesis and proliferation in primary cultures of adult rat hepatocytes. The results showed that phenylephrine (10?6 M) and metaproterenol (10?6 M) alone did not induce hepatocyte DNA synthesis and proliferation. However, when combined with l-ascorbic acid (10?6 M), these adrenoceptor agonists potentiated the hepatocyte DNA synthesis and proliferation induced by l-ascorbic acid. Then intracellular signal transduction mechanisms for the effects of phenylephrine and metaproterenol on l-ascorbic acid-induced hepatocyte mitogenesis were examined. Western blot analysis showed that phenylephrine and metaproterenol did not potentiate l-ascorbic acid-induced insulin-like growth factor I receptor tyrosine kinase phosphorylation. In contrast, they both significantly potentiated l-ascorbic acid-induced extracellular-signal regulated kinase-2 (ERK2) phosphorylation within 5 min. Moreover, cell-permeable second messenger analogs phorbol ester (10?7 M) and 8-bromo cAMP (10?7 M) mimicked the effects of phenylephrine and metaproterenol on l-ascorbic acid-induced ERK2 phosphorylation. The effects of these adrenoceptor agents were specifically antagonized by GF109203X and H-89, respectively. These results indicate that activation of ERK2 via protein kinas C and protein kinase A represents a mechanism for potentiation of l-ascorbic acid-induced hepatocyte DNA synthesis and proliferation in primary cultures of adult rat hepatocytes.
キーワード 注記 Author edition's title : Signal transduction mechanism for potentiation by α1- and β2-adrenergic agonists of L-ascorbic acid-induced DNA synthesis and proliferation in primary cultures of adult rat hepatocytes.
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