| ID | JOS-mco.2017.1287 |
| 著者：名前 | |
| 著者：別形式 | Oono, Keiichi / Takahashi, Katsuya / Sukehara, Saeka / Kurosawa, Hirohito / Matsumura, Tomio / Taniguchi, Shunichiro / Ohta, Shoichiro |
| 著者：カナ | |
| 著者：所属 | 城西大学薬学部臨床病理学研究室 / 城西大学薬学部臨床病理学研究室 / 城西大学薬学部臨床病理学研究室 / 城西大学薬学部臨床病理学研究室 / 信州大学先鋭領域融合研究群バイオメディカル研究所先端疾患予防学部門 / 信州大学医学部包括的がん治療学教室 / 城西大学薬学部臨床病理学研究室 |
| 著者：所属(別形式） | Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Pathology / Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Pathology / Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Pathology / Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Pathology / Shinshu University, Department of Advanced Medicine for Health Promotion, Interdisciplinary Cluster for Cutting Edge Research, Institute for Biomedical Sciences / Shinshu University, School of Medicine, Department of Comprehensive Cancer Therapy / Josai University, Faculty of Pharmaceutical Sciences, Department of Clinical Pathology |
| 著者版フラグ | publisher |
| 出版地 | Athens |
| 出版者 | Spandidos Publications |
| NCID | AA12610944 |
| 冊子ISSN | 20499450 |
| 電子ISSN | 20499469 |
| 掲載誌名 | |
| 巻 | 7 |
| 号 | 2 |
| 刊行年月 | 2017-08 |
| 開始ページ | 217 |
| 終了ページ | 220 |
| コンテンツ作成日 | 2016-07-08 |
| コンテンツ修正日 | 2017-04-06 |
| コンテンツ登録日 | 2018-03-12 |
| 識別番号：DOI | info:doi/10.3892/mco.2017.1287 |
| 識別番号：DOI（リンク） | |
| PubMed番号 | 28781788 |
| 抄録 | The n-3 fatty acids, eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA), found in fish oil, exert a number of beneficial effects, and they are used in the treatment of hyperlipidemia. In recent years, EPA and DHA have been found to affect cancer cell proliferation. In the present study, PC3 cells, which are androgen-independent prostate cancer cells that resemble castration-resistant prostate cancer cells, were used to investigate a possible novel treatment for castration-resistant prostate cancer. The PC3 cells were cultured and incubated with various concentrations of EPA or DHA. Cancer proliferation was confirmed by trypan blue microscopy. Invasion and migration assays were used in the upper chamber in PC3 cells, and serum-free medium and various concentrations of EPA or DHA were placed in the lower chamber in serum-containing medium. EPA and DHA decreased PC3 cell proliferation, invasion and migration. The effect of EPA on PC3 cells was dose-dependent and significant differences were observed at concentrations of 100 and 200 µg/ml. The effect of DHA on PC3 cells was similar to that of EPA. In the migration assay, EPA exerted almost no effects at 25 µg/ml, but migration was reduced at 50 µg/ml. Similar to EPA, DHA exerted almost no effects at 25 µg/ml, but further reduction was observed at the 50 µg/ml concentration. In the invasion assay, EPA at 25 µg/ml was not significantly different from the control, but suppressed invasion at 50 µg/ml. DHA decreased invasion compared with the control at 25 µg/ml, whereas invasion was significantly reduced at a DHA concentration of 50 µg/ml. In conclusion, it was demonstrated that EPA and DHA were effective in decreasing the proliferation, invasion and migration of prostate PC3 cancer cells. However, the detailed underlying mechanisms have not yet been fully elucidated. |
| キーワード | |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | Copyright © Spandidos Publications 2017 |
| このアイテムを表示する：本文(pdf) |  ( 575.9KB) ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
