| ID | JOS-0258851X-25-763 |
| 著者：名前 | 小野, 真那巳/ 菅藤, 歌織/ 植木, 淳一/ 島田, 亜季/ 若林, 英嗣/ Matsuta, Tomohiko/ 坂上, 宏/ Kumada, Hidefumi/ Hamada, Nobushiro/ Kitajima, Madoka/ Oizumi, Hiroshi/ Oizumi, Takaaki |
| 著者：別形式 | Ono, Manami / Kantoh, Kaori / Ueki, Jun-ichi / Shimada, Aki / Wakabayashi, Hidetsugu / Matsuta, Tomohiko / Sakagami, Hiroshi / Kumada, Hidefumi / Hamada, Nobushiro / Kitajima, Madoka / Oizumi, Hiroshi / Oizumi, Takaaki |
| 著者：カナ | オノ, マナミ/ カントウ, カオリ/ ウエキ, ジュンイチ/ シマダ, アキ/ ワカバヤシ, ヒデツグ/ マツタ, トモヒコ/ サカガミ, ヒロシ/ クマダ, ヒデフミ/ ハマダ, ノブシロウ/ キタジマ, マドカ/ オオイズミ, ヒロシ/ オオイズミ, タカアキ |
| 著者：所属 | 城西大学理学部 / 城西大学理学部 / 城西大学理学部 / 城西大学理学部 / 城西大学理学部 / 明海大学歯学部 / 明海大学歯学部 / 神奈川歯科大学 / 神奈川歯科大学 / 大和生物研究所 / 大和生物研究所 / 大和生物研究所 |
| 著者：所属(別形式） | Josai University, Faculty of Science / Josai University, Faculty of Science / Josai University, Faculty of Science / Josai University, Faculty of Science / Josai University, Faculty of Science / Meikai University School of Dentistry / Meikai University School of Dentistry / Kanagawa Dental College / Kanagawa Dental College / Daiwa Biological Research Institute / Daiwa Biological Research Institute / Daiwa Biological Research Institute |
| 著者版フラグ | publisher |
| 出版地 | Athens |
| 出版者 | International Institute of Anticancer Research |
| NCID | AA10714348 |
| 冊子ISSN | 0258851X |
| 掲載誌名 | |
| 巻 | 25 |
| 号 | 5 |
| 刊行年月 | 2011-09 |
| 開始ページ | 763 |
| 終了ページ | 768 |
| コンテンツ作成日 | 2011-06-03 |
| コンテンツ登録日 | 2012-03-15 |
| 抄録 | Background: We have previously reported that azulene-related compounds, and alkaline extract of Sasa senanensis Rehder potently inhibited nitric oxide (NO) production by lipopolysaccharide (LPS)-stimulated mouse macrophages. We investigated here whether they can inhibit pro-inflammatory cytokine production, by activated human gingival fibroblast (HGF). Materials and Methods: HGF was established from the periodontal tissues of extracted tooth. Viable cell number was determined by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) method. Production of Prostaglandin E2 (PGE2) and cytokines was determined by enzyme immunoassay, and enzyme-linked immunosorbent assay, respectively. Results: Interleukin (IL)-1β did not inhibit, but rather slightly stimulated the growth of HGF cells. IL-1β stimulated the production of PGE2, IL-6, IL-8 and monocyte chemotactic protein-1 very potently, but not that of nitric oxide and tumor necrosis factor-α. Native LPS and synthetic lipid A from E. coli and P. gingivalis was much less stimulatory. Dexamethasone, not indomethacin, was an efficient inhibitor of IL-8 production. Among five azulene-related compounds, benzo[b]cyclohepta[e][1,4]thiazine most potently inhibited the IL-8 production by HGF cells, as well as NO production by activated RAW264.7 cells. The alkaline extract of Sasa senanensis Rehder significantly inhibited IL-8 production, without affecting the cell viability. Conclusion: The present system may be applicable for use in the search for anti-gingivitis substances. |
| キーワード | |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| このアイテムを表示する：本文(pdf) |  ( 706.7KB) ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
