| ID | JOS-BSR20210304 |
| 著者：名前 | |
| 著者：別形式 | Nomura, Kaho / Kimira, Yoshifumi / Osawa, Yoshihiro / Kataoka-Matsushita, Aya / Takao, Koichi / Sugita, Yoshiaki / Shimizu, Jun / Wada, Masahiro / Mano, Hiroshi |
| 著者：カナ | |
| 著者：所属 | 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 新田ゼラチン株式会社 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 |
| 著者：所属(別形式） | Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Nitta Gelatin Inc. / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmaceutical Sciences |
| 著者版フラグ | publisher |
| 出版地 | London |
| 出版者 | Portland Press |
| 冊子ISSN | 01448463 |
| 電子ISSN | 15734935 |
| 掲載誌名 | |
| 巻 | 41 |
| 号 | 12 |
| 刊行年月 | 2021-12 |
| コンテンツ作成日 | 2021-02-03 |
| コンテンツ修正日 | 2021-11-10 |
| コンテンツ登録日 | 2022-02-28 |
| 識別番号：DOI | info:doi/10.1042/BSR20210304 |
| 識別番号：DOI（リンク） | |
| PubMed番号 | 34779485 |
| 抄録 | Collagen-derived dipeptide prolyl-hydroxyproline (Pro-Hyp) directly binds to the forkhead box g1 (Foxg1) protein and causes it to undergo structural alteration. Pro-Hyp also promotes the production of a regulator of osteoblast differentiation, Runt-related transcription factor 2 (Runx2), through Foxg1, inducing osteoblast differentiation. In addition, Pro-Hyp disrupts the interaction between Foxg1 and Runx2, and Foxg1 appears to interact with Runx2 in the absence of Pro-Hyp. To elucidate the mechanism of Pro-Hyp that promotes osteoblast differentiation, we investigated whether Pro-Hyp regulates the Runx2 P1 promoter together with Foxg1. The present study revealed that Pro-Hyp is taken up by osteoblastic cells via the solute carrier family 15 member (Slc15a) 4. In the presence of Pro-Hyp, Runx2 is translocated from the nucleus to the cytoplasm and Foxg1 is translocated from the cytoplasm to the nucleus. We also found that Pro-Hyp promoted the interaction between Forkhead box o1 (Foxo1) and Runx2 and the dissociation of Foxg1 from Runx2. Moreover, we identified the Pro-Hyp response element in the Runx2 distal P1 promoter at nt -375 to -316, including the Runx2 binding sites and Fox core sequence. In the presence of Pro-Hyp, Runx2 is dissociated from the Pro-Hyp response element in the Runx2 distal P1 promoter. Subsequently, Foxg1 and Foxo1 activated the Runx2 promoter by binding to the Pro-Hyp response element. In summary, we delineated the mechanism by which Pro-Hyp stimulates the bone-related Runx2 distal P1 promoter activity in osteoblastic cells through Foxg1, Foxo1, and Runx2. |
| キーワード | |
| 注記 | 16p. Article No.BSR20210304 This is an open access article published by Portland Press Limited on behalf of the Biochemical Society and distributed under the Creative Commons Attribution License 4.0 (CC BY). |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | Copyright © 2021 The Author(s). |
| このアイテムを表示する：本文(pdf) |  ( 1.5MB) ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
