| ID | JOS-fphar.2026.1718569 |
| 著者：名前 | |
| 著者：別形式 | 騎馬, 由佳 / 林, 豪士 / 安藤, 広和 / 鎌内, 等 / 横川, 貴美 / 鈴木, 龍一郎 / 谷川, 尚 / 北村, 雅史 |
| 著者：カナ | |
| 著者：所属 | Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / National Institute of Infectious Diseases, Japan Institute for Health Security, Department of Virology II. / Kanazawa University, Faculty of Pharmacy, Institute of Medical, Pharmaceutical and Health Sciences / Musashino University, Research Institute of Pharmaceutical Sciences / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Ritsumeikan University, College of Pharmaceutical Sciences ; Josai University, Faculty of Pharmacy and Pharmaceutical Sciences |
| 著者：所属(別形式） | 城西大学薬学部 / 国立健康危機管理研究機構国立感染症研究所ウイルス第Ⅱ部 / 金沢大学医薬保健学域薬学類 / 武蔵野大学薬学研究所 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 立命館大学薬学部 ; 城西大学薬学部 |
| 著者版フラグ | publisher |
| 出版地 | Lausanne |
| 出版者 | Frontiers Media |
| 電子ISSN | 16639812 |
| 掲載誌名 | |
| 巻 | 17 |
| 号 | 2026 |
| 刊行年月 | 2026-07 |
| 開始ページ | 1 |
| 終了ページ | 10 |
| コンテンツ作成日 | 2026-06-11 |
| コンテンツ登録日 | 2026-07-08 |
| 識別番号：DOI | info:doi/10.3389/fphar.2026.1718569 |
| 識別番号：DOI（リンク） | |
| 抄録 | Introduction: Maoto (麻黄湯) is a traditional Japanese Kampo formula composed of four crude drugs—Ephedrae Herba, Armeniacae Semen, Cinnamomi Cortex, and Glycyrrhizae Radix—and has been used to treat febrile viral illnesses. Among these, Ephedrae Herba has been associated with antiviral activity in previous studies. However, little is known about the effects of Ephedrae Herba on viral infection, spike (S) protein-mediated syncytium formation (cell–cell fusion), and viral enzymes, or the specific metabolites underlying these activities. In this study, we focused on Ephedrae Herba and comparatively evaluated the effects of the four crude drugs of Maoto on SARS-CoV-2 infection, S protein-mediated cell–cell fusion, and virus replication-related processes.
Methods: We evaluated the inhibitory effects of the four crude drugs of Maoto on SARS-CoV-2 infection in VeroE6/TMPRSS2 cells. S protein-mediated cell–cell fusion was evaluated using a split-luciferase assay in HEK293T cells. The inhibitory effects on viral enzymes, including 3C-like protease, papain-like protease, and RNA-dependent RNA polymerase (RdRp), were assessed using in vitro enzymatic assays.
Results: Comparative analyses of the four crude drugs showed that Ephedrae Herba, Armeniacae Semen, and Cinnamomi Cortex suppressed SARS-CoV-2 infection in VeroE6/TMPRSS2 cells, whereas Glycyrrhizae Radix inhibited S protein-mediated cell–cell fusion in HEK293T cells. In the biochemical assays, Ephedrae Herba and Cinnamomi Cortex showed inhibitory activity against SARS-CoV-2 RdRp. Further analysis of Ephedrae Herba showed that its high-polarity fraction exhibited concentration-dependent inhibitory activity against both SARS-CoV-2 and norovirus RdRp with IC50 values of 8.6 and 16.5 μg/mL, respectively.
Conclusion: Ephedrae Herba contains high-polarity metabolites associated with RdRp-related inhibitory activity in biochemical assays. Nevertheless, further studies are needed to determine the extent to which this activity contributes to the effects observed in cells. |
| キーワード | |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | ©2026 Frontiers Media |
| このアイテムを表示する：本文(pdf) | |
| このアイテムを表示する：URI | |
