| ID | JOS-j.jbc.2023.104600 |
| 著者：名前 | |
| 著者：別形式 | Yokokawa, Reo / Watanabe, Kana / Kanda, Shinji / Nishino, Yoshihide / Yasumasu, Shigeki / Sano, Kaori |
| 著者：カナ | |
| 著者：所属 | 城西大学理学研究科物質科学専攻 / 城西大学薬学研究科 / 東京大学大気海洋研究所 / 上智大学理工学部物質生命理工学科 / 上智大学理工学部物質生命理工学科 / 城西大学理学部化学科 |
| 著者：所属(別形式） | Josai University, Graduate School of Science, Department of Materials Science / Josai University, Graduate School of Pharmaceutical Sciences / The University of Tokyo, Atmosphere and Ocean Research Institute / Sophia University, aculty of Science and Technology, Materials and Lifesciences / Sophia University, aculty of Science and Technology, Materials and Lifesciences / Josai University, Faculty of Science, Department of Chemistry |
| 著者版フラグ | publisher |
| 出版者 | Elsevier |
| 冊子ISSN | 00219258 |
| 電子ISSN | 1083351X |
| 掲載誌名 | |
| 巻 | 299 |
| 号 | 4 |
| 刊行年月 | 2023-04 |
| 開始ページ | 1 |
| 終了ページ | 14 |
| コンテンツ作成日 | 2022-11-26 |
| コンテンツ登録日 | 2023-05-31 |
| 識別番号：DOI | info:doi/10.1016/j.jbc.2023.104600 |
| 識別番号：DOI（リンク） | |
| PubMed番号 | 36906145 |
| 抄録 | Teleost oocytes are surrounded by a structure called chorion or egg envelopes, which is composed of zona pellucida (ZP) proteins. As a result of the gene duplication in teleost, the expression site of the zp genes, coding the major component protein of egg envelopes, changed from the ovary to the maternal liver. In Euteleostei, there are three liver-expressed zp genes, named choriogenin (chg) h, chg hm, and chg l, and the composition of the egg envelope is mostly made up of these Chgs. In addition, ovary-expressed zp genes are also conserved in the medaka genomes, and their proteins have also been found to be minor components of the egg envelopes. However, the specific role of liver-expressed versus ovary-expressed zp genes was unclear. In the present study, we showed that ovary-synthesized ZP proteins first form the base layer of the egg envelope and then Chgs polymerize inwardly to thicken the egg envelope. To analyze the effects of dysfunction of the chg gene, we generated some chg knockout medaka. All knockout females failed to produce normally fertilized eggs by the natural spawning. The egg envelopes lacking Chgs were significantly thinner, but layers formed by ZP proteins synthesized in the ovary were found in the thin egg envelope of knockout as well as wildtype eggs. These results suggest that the ovary-expressed zp gene is well conserved in all teleosts, including those species in which liver-derived ZP proteins are the major component, because it is essential for the initiation of egg envelope formation. |
| キーワード | |
| 注記 | p.14 Article number: 104600 Published by Elsevier Inc on behalf of American Society for Biochemistry and Molecular Biology. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | Copyright © 2023 THE AUTHORS. |
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| このアイテムを表示する：URI | |
