城西大学機関リポジトリJURARapid identification of Colchicum autumnale based on loop-mediated isothermal amplification (LAMP) assay

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Rapid identification of Colchicum autumnale based on loop-mediated isothermal amplification (LAMP) assay

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ID	JOS-s11419-020-00557-4
著者：名前	Misawa, Sae/Natsuhara, Daigo/Kiba, Yuka/Yamamuro, Tadashi/Suzuki, Ryuichiro/Shibata, Takayuki/Kitamura, Masashi
著者：別形式	見沢, 沙瑛 / 夏原, 大悟 / 騎馬, 由佳 / 山室, 匡史 / 鈴木, 龍一郎 / 柴田, 隆行 / 北村, 雅史
著者：カナ	ミサワ, サエ/ナツハラ, ダイゴ/キバ, ユカ/ヤマムロ, タダシ/スズキ, リュウイチロウ/シバタ, タカユキ/キタムラ, マサシ
著者：所属	Josai University Laboratory of Pharmacognocy, School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences / Toyohashi University of Technology Department of Mechanical Engineering / School of Pharmacy Laboratory of Pharmacognocy / National Research Institute of Police Science of Pharmacy ; Josai University Pharmaceutical Sciences / Josai University Laboratory of Pharmacognocy, School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences / Toyohashi University of Technology Department of Mechanical Engineering / Josai University Laboratory of Pharmacognocy, School of Pharmacy, Faculty of Pharmacy and Pharmaceutical Sciences
著者：所属(別形式）	城西大学薬学部生薬学研究室 / 豊橋技術科学大学機械工学科 / 城西大学薬学部生薬学研究室 / 科学警察研究所 / 城西大学薬学部生薬学研究室 / 豊橋技術科学大学機械工学科 / 城西大学薬学部生薬学研究室
著者版フラグ	author
出版地	Tokyo
出版者	Springer Japan
NCID	AA12435006
冊子ISSN	18608965
掲載誌名	Forensic toxicology
巻	39
刊行年月	2020-11
開始ページ	259
終了ページ	365
コンテンツ作成日	2020-10-18
コンテンツ登録日	2026-05-27
識別番号：DOI	info:doi/10.1007/s11419-020-00557-4
識別番号：DOI（リンク）	https://doi.org/10.1007/s11419-020-00557-4
抄録	Purpose　Colchicum autumnale (Colchicaceae) exhibits toxicity, and severe poisoning cases due to ingestion of this plant have been reported in Japan. Identifying the cause of poisoning is important for emergency medical treatment, and a rapid and simple detection technique is required for the identification of the cause of poisoning. In the present study, we developed a rapid and simple method for the detection of C. autumnale based on a loop-mediated isothermal amplification (LAMP) assay, which is 100- to 1000-fold higher than the conventional polymerase chain reaction method. Methods　Specific LAMP primers for C. autumnale were designed based on the trnL-trnF intergenic spacer region. Using the LAMP primers, the LAMP assay was performed at an isothermal reaction temperature of 63 ℃. Results　The LAMP reaction was shown to be specific and highly sensitive to C. autumnale, given that the assay can be used for 10 pg of purified DNA. Using a simple protocol for on-site detection, the entire procedure from pretreatment to evaluation required approximately 1 h. Moreover, the LAMP method using a microfluidic device detected multiple genes, including C. autumnale-specific DNA regions, at a time. Conclusions　The currently proposed protocol exhibits good potential as a screening method for C. autumnale poisoning in emergency medical care.
キーワード	Colchicum/Isothermal DNA amplification/LAMP/Identification of toxic plants/Colchicum autumnale/On-site identification
言語	eng
資源タイプ	text
ジャンル	01学術雑誌論文 / Journal Article
フォーマット	application/pdf
権利	©2020 Japanese Association of Forensic Toxicology
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このアイテムを表示する：URI	https://libir.josai.ac.jp/il/meta_pub/G0000284repository_JOS-s11419-020-00557-4

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