ID JOS-s41598-021-92934-1
著者:名前 著者:別形式 Osawa, Yoshihiro / Nomura, Kaho / Kimira, Yoshifumi / Kushibe, Seiji / Takeyama, Ken-ichi / Nagao, Masashi / Kataoka-Matsushita, Aya / Koizumi, Seiko / Mano, Hiroshi
著者:カナ 著者:所属 城西大学薬学部医療栄養学科 / 城西大学薬学部医療栄養学科 / 城西大学薬学部医療栄養学科 / 城西大学経営学部 マネジメント総合学科/ 東京農業大学農生命科学研究所 / 順天堂大学大学院スポーツ健康科学研究科 / 新田ゼラチン株式会社 / 新田ゼラチン株式会社 / 城西大学薬学部医療栄養学科
著者:所属(別形式) Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Department of Clinical Dietetics and Human Nutrition / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Department of Clinical Dietetics and Human Nutrition / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Department of Clinical Dietetics and Human Nutrition / Josai University, Faculty of Management, Department of Management / Tokyo University of Agriculture, Research Institute of Agriculture and Life Sciences / Juntendo University, Graduate School of Health and Sports Science / Nitta Gelatin Inc, / Nitta Gelatin Inc, / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Department of Clinical Dietetics and Human Nutrition
著者版フラグ publisher
出版者 Nature Research
電子ISSN 20452322
掲載誌名 巻 11
号 1
刊行年月 2021-06
開始ページ 1
終了ページ 8
コンテンツ作成日 2021-02-25
コンテンツ修正日 2021-06-08
コンテンツ登録日 2021-09-21
識別番号:DOI info:doi/10.1038/s41598-021-92934-1
識別番号:DOI(リンク) PubMed番号 34188133
抄録 Active collagen oligopeptides (ACOP) are bioactive collagen-derived peptides detected by a recently-established ELISA. To facilitate studies of the function and metabolism of these products, this study aims to determine which of these peptides is recognized by a novel anti-ACOP antibody used in this ELISA. We then investigate the effect of collagen peptide (CP) ingestion and exercise on urinary ACOP concentrations in a cohort of university student athletes using colorimetric, LC–MS/MS, and ELISA. We observed that the antibody showed strong cross-reactivity to Pro-Hyp and Gly-Pro-Hyp and weak cross-reactivity to commercial CP. CP ingestion increased the urinary level of ACOP over time, which correlated highly with urinary levels of peptide forms of Hyp and Pro-Hyp. Physical activity significantly decreased the urinary ACOP level. This study demonstrates changes in urinary ACOP following oral CP intake and physical activity using ELISA with the novel anti-ACOP antibody. Thus, ACOP may be useful as a new biomarker for collagen metabolism.
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