| ID | JOS-s41598-022-16150-1 |
| 著者：名前 | |
| 著者：別形式 | Sano,Kaori / Uzawa, Yuya / Kaneshima, Itsuki / Nakasato, Saika / Hashimoto, Masashi / Tanaka, Yoshiyuki / Nakatani, Sachie / Kobata, Kenji |
| 著者：カナ | |
| 著者：所属 | 城西大学理学部化学科 / 城西大学理学部化学科 / 城西大学薬学研究科 / 城西大学薬学研究科 / 城西大学理学部化学科 / 京都大学農学研究科 / 城西大学薬学研究科 / 城西大学薬学研究科 |
| 著者：所属(別形式） | Josai University, Faculty of Science, Department of Chemistry / Josai University, Faculty of Science, Department of Chemistry / Josai University, Graduate School of Pharmaceutical Sciences / Josai University, Graduate School of Pharmaceutical Sciences / Josai University, Faculty of Science, Department of Chemistry / Kyoto University, Graduate School of Agriculture / Josai University, Graduate School of Pharmaceutical Sciences / Josai University, Graduate School of Pharmaceutical Sciences |
| 著者版フラグ | publisher |
| 出版者 | SpringerNature |
| 電子ISSN | 20452322 |
| 掲載誌名 | |
| 巻 | 12 |
| 刊行年月 | 2022-07 |
| 開始ページ | 1 |
| 終了ページ | 13 |
| コンテンツ作成日 | 2022-04-20 |
| コンテンツ修正日 | 2022-07-05 |
| コンテンツ登録日 | 2022-10-31 |
| 識別番号：DOI | info:doi/10.1038/s41598-022-16150-1 |
| 識別番号：DOI（リンク） | |
| 抄録 | Capsicum fruits synthesize capsaicin from vanillylamine, which is produced from vanillin in a reaction catalyzed by a putative aminotransferase (pAMT). Capsiate, a non-pungent compound that is structurally similar to capsaicin, is synthesized from vanillyl alcohol rather than vanillylamine. Vanillyl alcohol is possibly generated by the enzymatic reduction of vanillin, but the enzyme responsible for this reaction is unknown. In the present study, we revealed that the vanillin reductase in the capsiate biosynthetic pathway is cinnamyl alcohol dehydrogenase (CAD), which is an enzyme involved in lignin synthesis. The reduction of vanillin to vanillyl alcohol was greater in the mature red fruit placental extract than in the immature green fruit placental extract. This reduction was suppressed by both N-(O-hydroxyphenyl) sulfinamoyltertiobutyl acetate, a specific inhibitor of CAD, and ethylenediaminetetraacetic acid, a metalloenzyme inhibitor. The CaCAD1 transcript levels in the placenta were higher in the red fruits than in the green fruits. A recombinant CaCAD1 protein obtained using an Escherichia coli expression system reduced vanillin to vanillyl alcohol. This reaction was suppressed by the CAD inhibitors. These results strongly suggest that CAD is the enzyme that catalyzes the reduction of vanillin to vanillyl alcohol during capsiate biosynthesis. Syntenic analyses indicated that genes encoding CAD and capsaicin synthase (Pun1) involved in capsiate biosynthesis were acquired before the pAMT gene during the evolution of the family Solanaceae. This raises the possibility that in the genus Capsicum, the capsiate biosynthetic pathway emerged before the pAMT-encoding gene was acquired as the final trigger for capsaicin biosynthesis. |
| キーワード | |
| 注記 | Article No.12384. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/. |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | Copyright © The Author(s) 2022 |
| このアイテムを表示する：本文(pdf) |  ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
