| ID | JOS-s41598-023-30180-3 |
| 著者：名前 | |
| 著者：別形式 | Itakura, Shoko / Shohji, Ayaka / Amagai, Sayaka / Kitamura, Masashi / Takayama, Kozo / Sugibayashi, Kenji / Todo, Hiroaki |
| 著者：カナ | |
| 著者：所属 | 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西大学薬学部 / 城西国際大学薬学部 / 城西大学薬学部 |
| 著者：所属(別形式） | Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Josai International University, Faculty of Pharmaceutical Sciences / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences |
| 著者版フラグ | publisher |
| 出版地 | London |
| 出版者 | Nature Research |
| 電子ISSN | 20452322 |
| 掲載誌名 | |
| 巻 | 13 |
| 号 | 1 |
| 刊行年月 | 2023-02 |
| 開始ページ | 1 |
| 終了ページ | 13 |
| コンテンツ作成日 | 2022-10-21 |
| コンテンツ修正日 | 2023-02-17 |
| コンテンツ登録日 | 2023-05-31 |
| 識別番号：DOI | info:doi/10.1038/s41598-023-30180-3 |
| 識別番号：DOI（リンク） | |
| PubMed番号 | 36813850 |
| 抄録 | Small interfering RNAs (siRNAs) knockdown the expression of target genes by causing mRNA degradation and are a promising therapeutic modality. In clinical practice, lipid nanoparticles (LNPs) are used to deliver RNAs, such as siRNA and mRNA, into cells. However, these artificial nanoparticles are toxic and immunogenic. Thus, we focused on extracellular vesicles (EVs), natural drug delivery systems, for the delivery of nucleic acids. EVs deliver RNAs and proteins to specific tissues to regulate various physiological phenomena in vivo. Here, we propose a novel method for the preparation siRNAs encapsulated in EVs using a microfluidic device (MD). MDs can be used to generate nanoparticles, such as LNPs, by controlling flow rate to the device, but the loading of siRNAs into EVs using MDs has not been reported previously. In this study, we demonstrated a method for loading siRNAs into grapefruit-derived EVs (GEVs), which have gained attention in recent years for being plant-derived EVs developed using an MD. GEVs were collected from grapefruit juice using the one-step sucrose cushion method, and then GEVs-siRNA-GEVs were prepared using an MD device. The morphology of GEVs and siRNA-GEVs was observed using a cryogenic transmission electron microscope. Cellular uptake and intracellular trafficking of GEVs or siRNA-GEVs to human keratinocytes were evaluated by microscopy using HaCaT cells. The prepared siRNA-GEVs encapsulated 11% of siRNAs. Moreover, intracellular delivery of siRNA and gene suppression effects in HaCaT cells were achieved using these siRNA-GEVs. Our findings suggested that MDs can be used to prepare siRNA-EV formulations. |
| キーワード | |
| 注記 | p.13 Article number: 3102 This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http:// creat iveco mmons. org/ licen ses/ by/4. 0/. |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | Copyright © The Author(s) 2023 |
| このアイテムを表示する：本文(pdf) |  ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
