| ID | JOS-glycob_cwz022 |
| 著者：名前 | |
| 著者：別形式 | Takeuchi, Tomoharu / Tamura, Mayumi / Ishiwata, Kenji / Hamasaki, Megumi / Hamano, Shinjiro / Arata, Yoichiro / Hatanaka, Tomomi |
| 著者：カナ | |
| 著者：所属 | 城西大学薬学部 / 帝京大学薬学部 / 東京慈恵会医科大学熱帯医学講座 / 長崎大学寄生虫学分野熱帯医学研究所 (熱研) / 長崎大学寄生虫学分野熱帯医学研究所 (熱研) / 帝京大学薬学部 / 城西大学薬学部 |
| 著者：所属(別形式） | Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Teikyo University, Faculty of Pharma-Science / The Jikei University School of Medicine, Department of Tropical Medicine / Nagasaki University, Department of Parasitology, Institute of Tropical Medicine (NEKKEN) / Nagasaki University, Department of Parasitology, Institute of Tropical Medicine (NEKKEN) / Teikyo University, Faculty of Pharma-Science / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences |
| 著者版フラグ | author |
| 出版者 | Oxford University Press |
| 電子ISSN | 14602423 |
| 掲載誌名 | |
| 巻 | 29 |
| 号 | 6 |
| 刊行年月 | 2019-06 |
| 開始ページ | 504 |
| 終了ページ | 512 |
| コンテンツ作成日 | 2019-02-02 |
| コンテンツ修正日 | 2019-03-13 |
| コンテンツ登録日 | 2020-07-21 |
| 識別番号：DOI | info:doi/10.1093/glycob/cwz022 |
| 識別番号：DOI（リンク） | |
| PubMed番号 | 30874734 |
| 抄録 | Galactoseβ1-4Fucose (GalFuc) is a unique disaccharide found in invertebrates including nematodes. A fungal galectin CGL2 suppresses nematode development by recognizing the galactoseβ1-4fucose epitope. The Caenorhabditis elegans galectin LEC-6 recognizes it as an endogenous ligand and the Glu67 residue of LEC-6 is responsible for this interaction. We found that mammalian galectin-2 (Gal-2) also has a comparable glutamate residue, Glu52. In the present study, we investigated the potential nematode-suppressing activity of Gal-2 using C. elegans as a model and focusing on Gal-2 binding to the GalFuc epitope. Gal-2 suppressed C. elegans development whereas its E52D mutant (Glu52 substituted by Asp), galectin-1 and galectin-3 had little effect on C. elegans growth. Lectin-staining using fluorescently-labeled Gal-2 revealed that, like CGL2, it specifically binds to the C. elegans intestine. Natural C. elegans glycoconjugates were specifically bound by immobilized Gal-2. Western blotting with anti-GalFuc antibody showed that the bound glycoconjugates had the GalFuc epitope. Frontal affinity chromatography with pyridylamine-labeled C. elegans N-glycans disclosed that Gal-2 (but not its E52D mutant) recognizes the GalFuc epitope. Gal-2 also binds to the GalFuc-bearing glycoconjugates of Ascaris and the GalFuc epitope is present in the parasitic nematodes Nippostrongylus brasiliensis and Brugia pahangi. These results indicate that Gal-2 suppresses C. elegans development by binding to its GalFuc epitope. The findings also imply that Gal-2 may prevent infestations of various parasitic nematodes bearing the GalFuc epitope. |
| キーワード | |
| 注記 | This is a pre-copyedited, author-produced version of an article accepted for publication in Glycobiology following peer review. The version of record is available online at: https://academic.oup.com/glycob/article/29/6/504/5381599 and DOI:10.1093/glycob/cwz022. |
| 言語 | eng |
| 資源タイプ | text |
| ジャンル | |
| フォーマット | application/pdf |
| 権利 | Copyright © The Author(s) 2019 |
| このアイテムを表示する：本文(pdf) |  ( 590.6KB) ダウンロード回数： 回 |
| このアイテムを表示する：URI | |
