ID | JOS-glycob_cwz022 |
著者:名前 | |
著者:別形式 | Takeuchi, Tomoharu / Tamura, Mayumi / Ishiwata, Kenji / Hamasaki, Megumi / Hamano, Shinjiro / Arata, Yoichiro / Hatanaka, Tomomi |
著者:カナ | |
著者:所属 | 城西大学薬学部 / 帝京大学薬学部 / 東京慈恵会医科大学熱帯医学講座 / 長崎大学寄生虫学分野熱帯医学研究所 (熱研) / 長崎大学寄生虫学分野熱帯医学研究所 (熱研) / 帝京大学薬学部 / 城西大学薬学部 |
著者:所属(別形式) | Josai University, Faculty of Pharmacy and Pharmaceutical Sciences / Teikyo University, Faculty of Pharma-Science / The Jikei University School of Medicine, Department of Tropical Medicine / Nagasaki University, Department of Parasitology, Institute of Tropical Medicine (NEKKEN) / Nagasaki University, Department of Parasitology, Institute of Tropical Medicine (NEKKEN) / Teikyo University, Faculty of Pharma-Science / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences |
著者版フラグ | author |
出版者 | Oxford University Press |
電子ISSN | 14602423 |
掲載誌名 | |
巻 | 29 |
号 | 6 |
刊行年月 | 2019-06 |
開始ページ | 504 |
終了ページ | 512 |
コンテンツ作成日 | 2019-02-02 |
コンテンツ修正日 | 2019-03-13 |
コンテンツ登録日 | 2020-07-21 |
識別番号:DOI | info:doi/10.1093/glycob/cwz022 |
識別番号:DOI(リンク) | |
PubMed番号 | 30874734 |
抄録 | Galactoseβ1-4Fucose (GalFuc) is a unique disaccharide found in invertebrates including nematodes. A fungal galectin CGL2 suppresses nematode development by recognizing the galactoseβ1-4fucose epitope. The Caenorhabditis elegans galectin LEC-6 recognizes it as an endogenous ligand and the Glu67 residue of LEC-6 is responsible for this interaction. We found that mammalian galectin-2 (Gal-2) also has a comparable glutamate residue, Glu52. In the present study, we investigated the potential nematode-suppressing activity of Gal-2 using C. elegans as a model and focusing on Gal-2 binding to the GalFuc epitope. Gal-2 suppressed C. elegans development whereas its E52D mutant (Glu52 substituted by Asp), galectin-1 and galectin-3 had little effect on C. elegans growth. Lectin-staining using fluorescently-labeled Gal-2 revealed that, like CGL2, it specifically binds to the C. elegans intestine. Natural C. elegans glycoconjugates were specifically bound by immobilized Gal-2. Western blotting with anti-GalFuc antibody showed that the bound glycoconjugates had the GalFuc epitope. Frontal affinity chromatography with pyridylamine-labeled C. elegans N-glycans disclosed that Gal-2 (but not its E52D mutant) recognizes the GalFuc epitope. Gal-2 also binds to the GalFuc-bearing glycoconjugates of Ascaris and the GalFuc epitope is present in the parasitic nematodes Nippostrongylus brasiliensis and Brugia pahangi. These results indicate that Gal-2 suppresses C. elegans development by binding to its GalFuc epitope. The findings also imply that Gal-2 may prevent infestations of various parasitic nematodes bearing the GalFuc epitope. |
キーワード | |
注記 | This is a pre-copyedited, author-produced version of an article accepted for publication in Glycobiology following peer review. The version of record is available online at: https://academic.oup.com/glycob/article/29/6/504/5381599 and DOI:10.1093/glycob/cwz022. |
言語 | eng |
資源タイプ | text |
ジャンル | |
フォーマット | application/pdf |
権利 | Copyright © The Author(s) 2019 |
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