ID | JOS-s11418-021-01519-9 |
著者:名前 | |
著者:別形式 | Kiba, Yuka / Oyama, Rio / Misawa, Sae / Tanikawa, Takashi / Kitamura, Masashi / Suzuki, Ryuichiro |
著者:カナ | |
著者:所属 | 城西大学薬学部薬科学科生薬学研究室 / 城西大学薬学部薬科学科天然物化学研究室 / 城西大学薬学部薬科学科天然物化学研究室 / 城西大学薬学部薬学科栄養治療学研究室 / 城西大学薬学部薬科学科生薬学研究室 / 城西大学薬学部薬科学科天然物化学研究室 |
著者:所属(別形式) | Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, School of Pharmacy, Laboratory of Pharmacognocy / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Department of Pharmaceutical Sciences, Laboratory of Natural Products and Phytochemistry / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Department of Pharmaceutical Sciences, Laboratory of Natural Products and Phytochemistry / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, School of Pharmacy, Laboratory of Nutri-Pharmacotherapeutics Management / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, School of Pharmacy, Laboratory of Pharmacognocy / Josai University, Faculty of Pharmacy and Pharmaceutical Sciences, Department of Pharmaceutical Sciences, Laboratory of Natural Products and Phytochemistry |
著者版フラグ | author |
出版者 | Springer |
冊子ISSN | 13403443 |
電子ISSN | 18610293 |
掲載誌名 | |
巻 | 75 |
号 | 4 |
刊行年月 | 2021-04 |
開始ページ | 1080 |
終了ページ | 1085 |
コンテンツ作成日 | 2021-02-19 |
コンテンツ修正日 | 2021-04-16 |
コンテンツ登録日 | 2022-08-31 |
識別番号:DOI | info:doi/10.1007/s11418-021-01519-9 |
識別番号:DOI(リンク) | |
PubMed番号 | 33928494 |
抄録 | The spike (S) protein of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) contains a cleavage motif R-X-X-R for furin-like enzymes at the boundary of the S1/S2 subunits. The cleavage of the site by cellular proteases is essential for S protein activation and virus entry. We screened the inhibitory effects of crude drugs on in vitro furin-like enzymatic activities using a fluorogenic substrate with whole-cell lysates. Of the 124 crude drugs listed in the Japanese Pharmacopeia, aqueous ethanolic extract of Cnidii Monnieris Fructus, which is the dried fruit of Cnidium monnieri Cussion, significantly inhibited the furin-like enzymatic activities. We further fractionated the plant extract and isolated the two active compounds with the inhibitory activity, namely, imperatorin and osthole, whose IC50 values were 1.45 mM and 9.45 µM, respectively. Our results indicated that Cnidii Monnieris Fructus might exert inhibitory effects on furin-like enzymatic activities, and that imperatorin and osthole of the crude drug could be potential inhibitors of the motif cleavage. |
キーワード | |
言語 | eng |
資源タイプ | text |
ジャンル | |
フォーマット | application/pdf |
権利 | Copyright © The Japanese Society of Pharmacognosy 2021 |
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